Yesterday I checked on the observation hive queen to see if she had started to lay. Unfortunately, I could find no eggs, brood or sign of her. We have had a lot of severe weather here in the Atlanta area over the last several days, so there is a good possibility that she either got lost and perished in a storm or was eaten by a bird. In any event it is back to square one with the observation hive. I have inserted a frame that has a queen cell that I harvested from another of my hives. The beecam is now focused on that so we will see what happens there.
On a brighter note, I checked the mating nucs at the river and found 10 of the 12 nucs had brood. I actually found and marked 9 of the 12 queens with a beautiful, distinctive fluorescent green dot on their thorax. In the two that did not have brood, I inserted a frame of very young brood from another established colony, so if in fact they are queenless the colony can attempt to raise a new queen of their own.